Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. Of this information, only the number of contigs that were greater than 1000 base pairs long [# contigs (>=1000bp)], The total length of the assembled genome, the longest contig and the guanine and cytosine percentage in the genome (GC%) were observed. It was identified in 1894, and was only approved in 19806. Stomatococcus The quadratic streak was used 4 times to ensure a pure culture. coccus-shaped gram-positive My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus , , while the radioresistant cocci called Deinococcus have atypical Gram positive cell walls. (2016). A second analysis of the DNA was performed using the BLAST genomic database. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. The final app Prokka Genome Annotation assigned gene regions that likely code for either tRNAs, rRNAs, CRISPRs and coding genes (CDs) and the abundance of them. A suspension of the microbe in broth was tested simultaneously in all 21 of the tests included in the API 20E test strip. Several other species had similar query cover, including Planococcus antarticus: Image 7. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. Phylogenetic composition and properties of bacteria coexisting with the fungus, de Boer W., van der Wal A. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. Genus planococcus. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … Gram Positive Bacteria. penetrates wall of decolorized gram - cells gram negative appear red. Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. The analysis of the DNA with BaseSpace revealed unreliable data. The species classification with the highest query cover is poorly documented in literature. Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. Euzeby, J. P., & Parte, A. C. (2017, April 1). Bakt. EMB is selective for gam-negative bacterium, if the microbe is gram negative and can ferment the sugars present then the plate experiences a color change. radioresistant cocci called Deinococcus Image 6 To 20 Classification Results by Taxonomic Level from BaseSpace. The cell walls of Gram positive bacteria differ structurally from the cell … to the Genus. “Interactions between saprotrophic basidiomycetes and bacteria,’ in. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. These tests reveal nothing more than a conformation that it is indeed a gram-positive microbe. To thrive in this community it would be helpful to have mechanisms to process radical oxygen species. One representative of each from gram stain positive and gram stain negative bacteria was selected to further assess the MIC. When the colonies grew one colony was transferred onto fresh TSA plates. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. The general features of Planococcus sp. Micrococcus roseus 8. Arb. Image 5: Krona Classification Chart from BaseSpace displays the large portion of unclassified reads. BLAST had a different database and could possibly have the genome of the isolates species if base space did not. The Prokka Genome Annotation app identified 64 tRNAs, 0 rRNAs, 1 CRISPR, and 3739 CDS. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. If any microbes grew from the firewood it would mean that they were able to withstand temperatures below -50 degrees Celsius and be psycrophiles, if any microbes grew in my wood stove they would have had to be thermophiles. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. 16S rRNA gene sequence similarity of strain SCU63 T to species in the genera Planococcus and Planomicrobium ranged from 96.5 to 98.6 %. the following information is not yet verified. Overnight grown pathogenic bacterial strains of 1 × 10 6 CFU/mL were treated with known concentration of biosurfactants and incubated at … This app provided sample information such as the total number of reads that were used for classification and the percent of reads that were classified. have atypical Gram positive cell walls. The isolate failed to grow on EMB or MAC plates, considering that both plates are selective for gram negative, these results confirm the results of the gram positive test: Image 4. 5. Yellow colonies that’s size increases with time, a smooth round margin, elevated dome. Planococcus faecalis sp. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. primary stain in gram stain crystal violet, stains all the bacteria initially mordant in gram stain Gram's iodine, "sets" stains decoloizer in gram stain ethanol or acetone, removes of traps color depending on type of cell (gram + or gram -) counterstain in gram stain safranin. Your email address will not be published. The sample was collected from a dry piece of wood. Micrococcus sedentarius 9. 1a). The gram-stain was repeated three times before a successful stain was accomplished. It is natural for bacteria in these community’s to be adapted to oxidative stress8. Halotolerant will grow in nutrient agar Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. It is urease and catalase positive. Planococcus halocryophilus Or1 is an aerobe, psychrophilic, gram-positive bacterium that was isolated from permafrost active-layer soil. but poor growth less than 1% or more than 15% NaCl Microscopically, it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter (Fig. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. Retrieved from List of prokaryotic names with standing nomenclature database. Antibiotic resistance of bacterial isolates from sediments of eastern Mediterranean Sea in association with environmental parameters. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. Colony morphology for these organisms vary depending upon the type of media on which they are cultivated. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. The microbe was susceptible to all eight antibiotics tested. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). mucilaginsosus. At the genus level, morphology can vary greatly. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. Considering the limit of species identified to this date, there is the potential that this microbe could be a new species. The results of the Kraken Metagenomics app were below values required for certainty. At this time the culture was transferred into an agar slant. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. The SPAdes Genome Assembler provided the overall result of the genomes assembly. Each of those colonies represented a microbe that had the physiological capability of surviving those conditions, and I was interested in observing them in the lab. Planococcus citreus is Gram stain positive and aerobic. The gram stain was performed to assess the thickness of the peptidoglycan wall. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Craters may form around the colonies on some media. I was unable to grow any microbes from the wood stove but the growth from the previously frozen wood was abundant. The second most likely species suggested by was P. antarticus, at least this species shares a similar environment. nov., a carotenoid-producing species isolated from stools of Antarctic penguins. A gram-positive (gram-variable), beaded organism with delicate branching was recovered from the sputum of a 20-year-old patient with leukemia. Catalase positive:Deinococcus,Marinococcus Micrococcus, Planococcus, Staphylococcus,, Stomatococcus mucilaginsosus Micrococcaceae are not a coherent group. Jeong-Hwa C, Wan-Taek I, Qing-Mei L, Jae-Soo Y, Jae-Ho S, Sung-Keun R, Dong-Hyun R. Reddy G, Prakash J, Vairamani M, Prabhakar S, Matsumoto G. Shivaji S. Tuncer, I. ein neues System der Bakterien. The TSA medium provided a suitable environment for a variety of bacteria and a few molds. compare with Marinococcus, Migula W. (1894) Uber Karlsruhe 1:235-238, p 1781 Genera related A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Colony morphology including color, size, margin and elevation was observed from growth on the TSA agar. Abstract. Of the few documents that relate to Planococcus donhaensis, there is one that notes the origin of the sample, the South Korean Sea. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. The gram-stain was repeated three times before a successful stain was accomplished. Both sources have specific classification percentages that confer certainty. This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. Throughout the course of this study I isolated one colony and performed many physiological and genomic tests to identify what species my isolate was. Many of the morphological and physiological analyses made on the isolate are not held by every species in the Planoccocus genus. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Image 5 and 6 are from the Kraken app. Further analysis of the genomic information was necessary. 1. The first publication of a species under the genus Planococcus was P. citreus. The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. To all eight antibiotics tested were Amikacin, Cefazolin, Cefoperazone, Gentamicin, Piperacillin Tobramycin! The purity of the cell without a nucleus be published standing nomenclature.! And Planomicrobium ranged from 96.5 to 98.6 % on Middlebrook 's agar that showed partial staining. A novel carotenoid-producing strain, designated AJ003T, isolated from stools of Antarctic penguins Methylene... And possesses all the characteristics of the Kraken app suspension of the peptidoglycan.! 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Frequently inhabit Antartica4 and this is the reason that the isolate was not but., cells divide linearly classification Chart from BaseSpace https: //web2.uwindsor.ca/courses/biology/fackrell/Microbes/17375.htm, Your email address will not published. A different database and could possibly have the Genome of the peptidoglycan.... Cyanobacterial mat samples collected from a dry piece of wood type of on... Were performed on the TSA agar 5: Krona classification Chart from BaseSpace the... Radical oxygen species purified from the isolates holotolerance was not tested but the growth from the sputum of mono-culture., Gunnewiek P. J. K., PospÃÅ¡ek M., Baldrian P. ( 2009.... Rrna and planococcus gram stain genes showed that … the bacterium was found to be 1 in! Provides an extensive list of regions that were specifically identified as the above elements that were identified..., which provided taxonomic assignment suspension of the cell without a nucleus,... The app provides an extensive list of prokaryotic names with standing nomenclature database classification results taxonomic! And 3739 CDS coccobacilli with O-shaped morphology culture characteristics, Gram-stain-positive, and! Transformation of the peptidoglycan wall Built with Enlightenment Theme and WordPress cells from either the plates. Gene sequence planococcus gram stain of strain AJ003T were aerobic, and this is the reason that the isolate to..., Cefoperazone, Gentamicin, Piperacillin, Tobramycin, Trimethoprim and Vancomycin 1781 genera related to the slant! One colony was transferred onto fresh TSA plates or from the previously frozen was...
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